三维实验的灵敏度列表

Experiment Assignment Comment Relative S/N[%]
HNCO H(i)-N(i)-C’(i-1) <20 kD, above use 2H labeling 100
HNCA H(i)-N(i)-Ca(i)Ca(i-1) <20 kD, above use 2H labeling 50/15
HN(Co)CA H(i)-N(i)-Ca(i-1) <20 kD, above use 2H labeling 71
HN(CA)Co H(i)-N(i)-C’(i) <20 kD, above use 2H labeling 13/4
CBCA(Co)NH H(i)-N(i)-Ca(i-1)Cb(i-1) <20 kD, above use 2H labeling 13/9 a/b
HBHA(Co)NH H(i)-N(i)-Ha(i-1)Hb(i-1) <20 kD, above use 2H labeling 13/9 a/b
CBCANH,HNCACB H(i)-N(i)-Ca(i-1)Ca(i)Cb(i-1)Cb(i) <15 kD, above use 2H labeling 4/1.7 a/b(i), 1.3/0.5 a/b(i-1)
(H)CC(Co)NH-Tocsy H(i)-N(i)-Caliph(i-1) <15-20 kD, above use 2H labeling
H(CC)(Co)NH-Tocsy H(i)-N(i)-Caliph(i-1) <15-20 kD, above use 2H labeling
HCCH-Tocsy Haliph-Caliph <25 kD, sensitive, but tedious to analyze, combine with HCCONH type exp